Dental and Medical Problems

Dent Med Probl
Index Copernicus (ICV 2021) – 132.50
MEiN – 70 pts
CiteScore (2021) – 2.0
JCI (2021) – 0.5
Average rejection rate (2022) – 79.69%
ISSN 1644-387X (print)
ISSN 2300-9020 (online)
Periodicity – quarterly

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Dental and Medical Problems

2022, vol. 59, nr 4, October-December, p. 565–571

doi: 10.17219/dmp/145593

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

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Patil RS, Kalburgi NB, Koregol AC, Vinayaka AM, Puttarevanna T, Hannahson P. Effect of non-surgical periodontal therapy on the salivary levels of omentin-1, a novel adipokine biomarker in periodontitis: A clinico-biochemical study. Dent Med Probl. 2022;59(4):565–571. doi:10.17219/dmp/145593

Effect of non-surgical periodontal therapy on the salivary levels of omentin-1, a novel adipokine biomarker in periodontitis: A clinico-biochemical study

Ruchita Sunil Patil1,A,B,C,D,E,F, Nagaraj Balasaheb Kalburgi2,A,C,F, Arati Chanabasappa Koregol2,A,B,C,D,E,F, Ambujakshi Manjunatha Vinayaka2,C,D,E,F, Tejashwini Puttarevanna1,B, Puladas Hannahson1,B

1 Postgraduate student, Department of Periodontics, P.M.N.M. Dental College and Hospital, Bagalkot, India

2 Department of Periodontics, P.M.N.M. Dental College and Hospital, Bagalkot, India


Background. Biomarkers are emerging, advanced diagnostic tools for the assessment of periodontal disease progression. Omentin-1 is an anti-inflammatory adipocytokine, which has been observed and studied in the saliva of periodontitis patients. Non-surgical periodontal therapy (NSPT) is considered a vital part of periodontal disease treatment.
Objectives. The study aimed to evaluate the interventional effect of NSPT on the levels of salivary omentin1 in healthy (H) and chronic periodontitis (CP) patients.
Material and methods. A total of 60 participants were selected and equally divided into 2 groups (group A: H participants, group B: CP patients). After obtaining verbal and written consent, whole unstimulated saliva was collected from all participants and analyzed for omentin-1 levels using enzyme-linked immunosorbent assay (ELISA).
Results. Mean salivary omentin-1 levels were elevated and found to be significantly higher in group A (95.80 ±26.65) compared to group B (61.97 ±24.53). In group B, there was a substantial rise in omentin1 levels from baseline to the 6th week of follow-up (p < 0.001). Thus, NSPT had a positive influence on salivary omentin-1 levels in the treatment group.
Conclusions. Salivary omentin-1 levels may serve as diagnostic and prognostic indicators of periodontal disease progression, and may be used to assess therapeutic outcomes in periodontitis patients.

Key words

omentin-1, periodontitis, saliva, biomarker, non-surgical periodontal therapy

References (41)

  1. Doğan ŞB, Dede FÖ, Ballı U, Sertoğlu E. Levels of vaspin and omentin-1 in gingival crevicular fluid as potential markers of inflammation in patients with chronic periodontitis and type 2 diabetes mellitus. J Oral Sci. 2016;58(3):379–389. doi:10.2334/josnusd.15-0731
  2. Lecic J, Cakic S, Pavlovic OJ, et al. Different methods for subgingival application of chlorhexidine in the treatment of patients with chronic periodontitis. Acta Odontol Scand. 2016;74(6):502–507. doi:10.1080/00016357.2016.1206964
  3. Cobb CM. Clinical significance of non-surgical periodontal therapy: An evidence-based perspective of scaling and root planing. J Clin Periodontol. 2002;29 Suppl 2:6–16. PMID:12010523.
  4. Stathopoulou PG, Buduneli N, Kinane DF. Systemic biomarkers for periodontitis. Curr Oral Health Rep. 2015;2:218–226. doi:10.1007/s40496-015-0072-9
  5. Mahendra J, Mahendra L, Mugri MH, et al. Role of periodontal bacteria, viruses, and placental mir155 in chronic periodontitis and preeclampsia – A genetic microbiological study. Curr Issues Mol Biol. 2021;43(2):831–844. doi:10.3390/cimb43020060
  6. Biomarkers Definitions Working Group. Biomarkers and surrogate endpoints: Preferred definitions and conceptual framework. Clin Pharmacol Ther. 2001;69(3):89–95. doi:10.1067/mcp.2001.113989
  7. Giannobile WV, Beikler T, Kinney JS, Ramseier CA, Morelli T, Wong DT. Saliva as a diagnostic tool for periodontal disease: Current state and future directions. Periodontol 2000. 2009;50:52–64. doi:10.1111/j.1600-0757.2008.00288.x
  8. Nagler R, Bahar G, Shpitzer T, Feinmesser R. Concomitant analysis of salivary tumor markers – A new diagnostic tool for oral cancer. Clin Cancer Res. 2006;12(13):3979–3984. doi:10.1158/1078-0432.CCR-05-2412
  9. Baldini C, Giusti L, Ciregia F, et al. Proteomic analysis of saliva: A unique tool to distinguish primary Sjögren’s syndrome from secondary Sjögren’s syndrome and other sicca syndromes. Arthritis Res Ther. 2011;13(6):R194. doi:10.1186/ar3523
  10. Aqrawi LA, Jensen JL, Øijordsbakken G, et al. Signalling pathways identified in salivary glands from primary Sjögren’s syndrome patients reveal enhanced adipose tissue development. Autoimmunity. 2018;51(3):135–146. doi:10.1080/08916934.2018.1446525
  11. Selvarajan S, Perumalsamy R, Emmadi P, Thiagarajan R, Namasivayam A. Association between gingival crevicular fluid leptin levels and periodontal status – A biochemical study on Indian patients. J Clin Diagn Res. 2015;9(5):ZC48–ZC53. doi:10.7860/JCDR/2015/12335.5941
  12. Karthikeyan BV, Pradeep AR. Leptin levels in gingival crevicular fluid in periodontal health and disease. J Periodontal Res. 2007;42(4):300–304. doi:10.1111/j.1600-0765.2006.00948.x
  13. Nokhbehsaim M, Keser S, Nogueira AVB, et al. Beneficial effects of adiponectin on periodontal ligament cells under normal and regenerative conditions. J Diabetes Res. 2014;2014:796565. doi:10.1155/2014/796565
  14. Wang Z, Chen Z, Fang F, Qiu W. The role of adiponectin in periodontitis: Current state and future prospects. Biomed Pharmacother. 2021;137:111358. doi:10.1016/j.biopha.2021.111358
  15. Balli U, Bozkurt Dogan S, Ongoz Dede F, Sertoglu E, Keles GC. The levels of visceral adipose tissue-derived serpin, omentin-1 and tumor necrosis factor-α in the gingival crevicular fluid of obese patients following periodontal therapy. J Oral Sci. 2016;58(4):465–473. doi:10.2334/josnusd.16-0212
  16. Yamawaki H, Kuramoto J, Kameshima S, Usui T, Okada M, Hara Y. Omentin, a novel adipocytokine inhibits TNF-induced vascular inflammation in human endothelial cells. Biochem Biophys Res Commun. 2011;408(2):339–343. doi:10.1016/j.bbrc.2011.04.039
  17. Kazama K, Usui T, Okada M, Hara Y, Yamawaki H. Omentin plays an anti-inflammatory role through inhibition of TNF-α-induced superoxide production in vascular smooth muscle cells. Eur J Pharmacol. 2012;686(1–3):116–123. doi:10.1016/j.ejphar.2012.04.033
  18. Zhong X, Li X, Liu F, Tan H, Shang D. Omentin inhibits TNF-α-induced expression of adhesion molecules in endothelial cells via ERK/NF-κB pathway. Biochem Biophys Res Commun. 2012;425(2):401–406. doi:10.1016/j.bbrc.2012.07.110
  19. Hiramatsu-Ito M, Shibata R, Ohashi K, et al. Omentin attenuates atherosclerotic lesion formation in apolipoprotein E-deficient mice. Cardiovasc Res. 2016;110(1):107–117. doi:10.1093/cvr/cvv282
  20. Wu SS, Liang QH, Liu Y, Cui RR, Yuan LQ, Liao EY. Omentin-1 stimulates human osteoblast proliferation through PI3K/Akt signal pathway. Int J Endocrinol. 2013;2013:368970. doi:10.1155/2013/368970
  21. American Academy of Periodontology task force report on the update to the 1999 Classification of Periodontal Diseases and Conditions. J Periodontol. 2015;86(7):835–838. doi:10.1902/jop.2015.157001
  22. Allen JC Jr. Sample size calculation for two independent groups: A useful rule of thumb. Proc Singapore Healthc. 2011;20(2):138–140. doi:10.1177/201010581102000213
  23. Löe H. The gingival index, the plaque index and the retention index systems. J Periodontol. 1967;38(6):610–616. doi:10.1902/jop.1967.38.6.610
  24. Greene JC, Vermillion JR. The simplified oral hygiene index. J Am Dent Assoc. 1964;68(1):7–13. doi:10.14219/jada.archive.1964.0034
  25. Mühlemann HR, Son S. Gingival sulcus bleeding – A leading symptom in initial gingivitis. Helv Odontol Acta. 1971;15(2):107–113. PMID:5315729.
  26. Flink H, Tegelberg A, Lagerlöf F. Influence of the time of measurement of unstimulated human whole saliva on the diagnosis of hyposalivation. Arch Oral Biol. 2005;50(6):553–559. doi:10.1016/j.archoralbio.2004.10.015
  27. Podzimek S, Vondrackova L, Duskova J, Janatova T, Broukal Z. Salivary markers for periodontal and general diseases. Dis Markers. 2016;2016:9179632. doi:10.1155/2016/9179632
  28. Bagwe S, Gopalakrishnan D, Mehta V, Mathur A, Kapare K, DeshpandeA. GCF and serum levels of omentin in periodontal health and disease of diabetic and non-diabetic individuals: A comparative study. Indian J Dent Res. 2020;31(4):520–525. doi:10.4103/ijdr.IJDR_796_18
  29. Elsaid NH, Sadik NA, Ahmed NR, Fayez SE, Mohammed NAE. Serum omentin-1 levels in type 2 diabetic obese women in relation to glycemic control, insulin resistance and metabolic parameters. J Clin Transl Endocrinol. 2018;13:14–19. doi:10.1016/j.jcte.2018.05.003
  30. Aguirre F, Brown A, Cho NH, et al. IDF Diabetes Atlas. 6th ed. Brussels, Belgium: International Diabetes Federation; 2014.
  31. Senolt L, Polanská M, Filková M, et al. Vaspin and omentin: New adipokines differentially regulated at the site of inflammation in rheumatoid arthritis. Ann Rheum Dis. 2010;69(7):1410–1411. doi:10.1136/ard.2009.119735
  32. Luque-Ramírez M, Martínez-García MA, Montes-Nieto R, et al. Sexual dimorphism in adipose tissue function as evidenced by circulating adipokine concentrations in the fasting state and after an oral glucose challenge. Hum Reprod. 2013;28(7):1908–1918. doi:10.1093/humrep/det097
  33. de Souza Batista CM, Yang RZ, Lee MJ, et al. Omentin plasma levels and gene expression are decreased in obesity. Diabetes. 2007;56(6):1655–1661. doi:10.2337/db06-1506
  34. Proye M, Caton J, Polson A. Initial healing of periodontal pockets after a single episode of root planing monitored by controlled probing forces. J Periodontol. 1982;53(5):296–301. doi:10.1902/jop.1982.53.5.296
  35. Cercek JF, Kiger RD, Garrett S, Egelberg J. Relative effects of plaque control and instrumentation on the clinical parameters of human periodontal disease. J Clin Periodontol. 1983;10(1):46–56. doi:10.1111/j.1600-051x.1983.tb01266.x
  36. Sato K, Yoneyama T, Okamoto H, Dahlén G, Lindhe J. The effect of subgingival debridement on periodontal disease parameters and the subgingival microbiota. J Clin Periodontol. 1993;20(5):359–365. doi:10.1111/j.1600-051x.1993.tb00373.x
  37. Sarhat ER, Rmaid ZJ, Jabir TH. Changes of salivary interleukine-17, apelin, omentin and vaspin levels in normal subjects and diabetic patients with chronic periodontitis. Ann Trop Med & Pub Health. 2020;23(1):S404. doi:10.36295/ASRO.2020.23118
  38. Dandona P, Aljada A, Chaudhuri A, Mohanty P, Garg R. Metabolic syndrome: A comprehensive perspective based on interactions between obesity, diabetes, and inflammation. Circulation. 2005;111(11):1448–1154. doi:10.1161/01.CIR.0000158483.13093.9D
  39. Moreno-Navarrete JM, Catalán V, Ortega F, et al. Circulating omentin concentration increases after weight loss. Nutr Metab (Lond). 2010;7:27. doi:10.1186/1743-7075-7-27
  40. Kabiri A, Hosseinzadeh-Attar MJ, Haghighatdoost F, Eshraghian M, Esmaillzadeh A. Impact of olive oil-rich diet on serum omentin and adiponectin levels: A randomized cross-over clinical trial among overweight women. Int J Food Sci Nutr. 2017;68(5):560–568. doi:10.1080/09637486.2016.1261808
  41. Wilms B, Ernst B, Gerig R, Schultes B. Plasma omentin-1 levels are related to exercise performance in obese women and increase upon aerobic endurance training. Exp Clin Endocrinol Diabetes. 2015;123(3):187–192. doi:10.1055/s-0034-1398504