Dental and Medical Problems

Dent Med Probl
Index Copernicus (ICV 2020) – 128.41
MEiN – 70 pts
CiteScore (2021) – 2.0
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ISSN 1644-387X (print)
ISSN 2300-9020 (online)
Periodicity – quarterly

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Dental and Medical Problems

2019, vol. 56, nr 2, April-June, p. 185–190

doi: 10.17219/dmp/105252

Publication type: review article

Language: English

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Creative Commons BY-NC-ND 3.0 Open Access

Extracted human teeth and their utility in dental research. Recommendations on proper preservation: A literature review

Zastosowanie usuniętych zębów ludzkich w badaniach naukowych. Wytyczne dotyczące przechowywania próbek – przegląd piśmiennictwa

Agnieszka Nawrocka1,A,B,C,D, Monika Łukomska-Szymańska1,A,E,F

1 Department of General Dentistry, Medical University of Lodz, Poland


Laboratory research in dentistry and dental education use extracted human teeth as a model for simulation for ex vivo procedures. Human-borne tissues are the first choice of material for bond strength assessment. To obtain comparable results in dental material tests and to ensure microbiological safety, specimens must be stored under specific, uniform conditions. The aim of this paper was to present the contemporary view and recommendations on preserving extracted human teeth. The antimicrobial properties of the storage medium are a crucial aspect, as extracted teeth pose a risk of cross-infection. A classification of different methods (using solutions and otherwise) is presented and their sterilizing efficiency is compared based on the literature. The emphasis is put on the interaction between the storage conditions and the substrate. Tooth specimens should be biologically safe and have normal mechanical properties. The sterilizing process must be neutral for the enamel and dentin microstructure, because even a minor change can affect the adhesive bonding. Autoclave sterilization and storage in 10% formalin solution are widespread and reliable methods, although they do have their disadvantages. There is a need for further investigation in order to establish uniform recommendations on preparing and preserving extracted human teeth used for research purposes.

Key words

sterilization, extracted teeth, formalin, autoclave, adhesion

Słowa kluczowe

sterylizacja, usunięte zęby, formalina, autoklaw, adhezja

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