Dental and Medical Problems

Dent Med Probl
Impact Factor (IF 2023) – 2.7
Journal Citation Indicator (JCI 2023) - 1.06
Scopus CiteScore (2023) – 4.0 (CiteScore Tracker – 4.9)
Index Copernicus (ICV 2023) – 181.00
MNiSW – 70 pts
ISSN 1644-387X (print)
ISSN 2300-9020 (online)
Periodicity – bimonthly


 

Download original text (EN)

Dental and Medical Problems

2019, vol. 56, nr 2, April-June, p. 143–148

doi: 10.17219/dmp/105408

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

Download citation:

  • BIBTEX (JabRef, Mendeley)
  • RIS (Papers, Reference Manager, RefWorks, Zotero)

The effect of dentin pre-treatment with activated riboflavin on the bond strength of a two-step self-etch adhesive system

Wpływ wstępnego pokrycia zębiny aktywowaną ryboflawiną na siłę wiązania dwustopniowego samotrawiącego systemu adhezyjnego

Shahin Kasraei1,A,E,F, Maryam Mojtahedi2,B,C,D, Mohammad-Taghi Goodarzi3,A,C,E, Mohadese Azarsina4,A,D,F, Zahra Khamverdi1,B,E

1 Department of Restorative Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 School of Dentistry, Hamadan University of Medical Sciences, Iran

3 Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Iran

4 Department of Operative Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Background. The cross-linking of collagen fibers in the hybrid layer has been suggested as a way to create more durable bonds.
Objectives. This study evaluated the effect of visible light-activated riboflavin (RF) as a cross-linking agent on the durability of the dentin microtensile bond strength (μTBS) in a 2-step self-etch (SE) adhesive system.
Material and Methods. The occlusal surfaces of 21 human premolar teeth were ground down to expose the dentin, and were then randomly divided into 3 groups. The Clearfil® SE Bond was used in the control group. In the RF/BL group, a 0.1 wt% aqueous solution of RF was applied to the dentin surface before applying the adhesive and irradiating with blue light (BL) for 2 min. In the 3rd group, the RF-P/BL group, the RF powder was added to the adhesive primer (P) at a concentration of 0.1 wt%. The teeth were built up using composite resin. After thermocycling, 14 resin–dentin beams from each group were prepared and stored in water for 3 months. The μTBS was determined and the data was analyzed using a linear model with a generalized estimating equation (GEE) (p = 0.05).
Results. The highest and the lowest μTBS belonged to the control group (41.15 ±3.50 MPa) and the RF-P/BL group (19.84 ±3.80 MPa), respectively. The mean μTBS in the control group was significantly higher than in the RF/BL and RF-P/BL groups (p < 0.001), but no significant difference was found between the RF/BL and RF-P/BL groups (p = 0.598).
Conclusion. Pre-treatment of dentin surfaces with RF activated with BL had a negative impact on the μTBS of the Clearfil SE Bond as a 2-step SE adhesive.

Key words

collagen, riboflavin, cross-linking agents, dentin bonding agents, photoactivation

Słowa kluczowe

kolagen, ryboflawina, środki sieciujące, zębinowe środki wiążące, fotoaktywacja

References (37)

  1. De Munck J, Van Landuyt K, Peumans M, et al. A critical review of the durability of adhesion to tooth tissue: Methods and results. J Dent Res. 2005;84(2):118–132.
  2. Perdigão J, Reis A, Loguercio AD. Dentin adhesion and MMPs: A comprehensive review. J Esthet Restor Dent. 2013;25(4):219–241.
  3. Mazzoni A, Pashley DH, Nishitani Y, et al. Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives. Biomaterials. 2006;27(25):4470–4476.
  4. Nishitani Y, Yoshiyama M, Wadgaonkar B, et al. Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives. Eur J Oral Sci. 2006;114(2):160–166.
  5. Mazzoni A, Carrilho M, Papa V, et al. MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: Biochemical and immunohistochemical analysis. J Dent. 2011;39(7):470–477.
  6. Mazzoni A, Nascimento FD, Carrilho M, et al. MMP activity in the hybrid layer detected with in situ zymography. J Dent Res. 2012;91(5):467–472.
  7. Hiraishi N, Yiu CK, King NM, Tay FR. Effect of chlorhexidine incorporation into a self-etching primer on dentine bond strength of a luting cement. J Dent. 2010;38(6):496–502.
  8. Stanislawczuk R, Pereira F, Muñoz MA, et al. Effects of chlorhexidine-containing adhesives on the durability of resin–dentine interfaces. J Dent. 2014;42(1):39–47.
  9. Cova A, Breschi L, Nato F, et al. Effect of UVA-activated riboflavin on dentin bonding. J Dent Res. 2011;90(12):1439–1445.
  10. Frassetto A, Breschi L, Turco G, et al. Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability – a literature review. Dent Mater. 2016;32(2):e41–e53.
  11. Seseogullari-Dirihan R, Tjäderhane L, Pashley DH, Tezvergil-Mutluay A. Effect of ultraviolet A-induced crosslinking on dentin collagen matrix. Dent Mater. 2015;31(10):1225–1231.
  12. Tjäderhane L, Nascimento FD, Breschi L, et al. Strategies to prevent hydrolytic degradation of the hybrid layer – a review. Dent Mater. 2013;29(10):999–1011.
  13. Bedran-Russo AK, Pashley DH, Agee K, Drummond JL, Miescke KJ. Changes in stiffness of demineralized dentin following application of collagen crosslinkers. J Biomed Mater Res B Appl Biomater. 2008;86(2):330–334.
  14. Walter R, Miguez PA, Arnold RR, Pereira PN, Duarte WR, Yamauchi M. Effects of natural cross-linkers on the stability of dentin collagen and the inhibition of root caries in vitro. Caries Res. 2008;42(4):263–268.
  15. Xu C, Wang Y. Cross-linked demineralized dentin maintains its mechanical stability when challenged by bacterial collagenase. J Biomed Mater Res B Appl Biomater. 2010;96(2):242–248.
  16. Daood U, Swee Heng C, Neo Chiew Lian J, Fawzy AS. In vitro analysis of riboflavin-modified, experimental, two-step etch-and-rinse dentin adhesive: Fourier transform infrared spectroscopy and micro-Raman studies. Int J Oral Sci. 2015;7(2):110–124.
  17. Fang M, Liu R, Xiao Y, et al. Biomodification to dentin by a natural crosslinker improved the resin–dentin bonds. J Dent. 2012;40(6):458–466.
  18. Spoerl E, Mrochen M, Sliney D, Trokel S, Seiler T. Safety of UVA-riboflavin cross-linking of the cornea. Cornea. 2007;26(4):385–389.
  19. Arbelaez MC, Sekito MB, Vidal C, Choudhury SR. Collagen cross-linking with riboflavin and ultraviolet-A light in keratoconus: One‑year results. Oman J Ophthalmol. 2009;2(1):33–38.
  20. McCall AS, Kraft S, Edelhauser HF, et al. Mechanisms of corneal tissue cross-linking in response to treatment with topical riboflavin and long-wavelength ultraviolet radiation (UVA). Invest Ophthalmol Vis Sci. 2010;51(1):129–138.
  21. Chiang YS, Chen YL, Chuang SF, et al. Riboflavin-ultraviolet-A-induced collagen cross-linking treatments in improving dentin bonding. Dent Mater. 2013;29(6):682–692.
  22. Seseogullari-Dirihan R, Apollonio F, Mazzoni A, et al. Use of crosslinkers to inactivate dentin MMPs. Dent Mater. 2016;32(3):423–432.
  23. Daood U, Iqbal K, Nitisusanta LI, Fawzy AS. Effect of chitosan/riboflavin modification on resin/dentin interface: Spectroscopic and microscopic investigations. J Biomed Mater Res A. 2013;101(7):1846–1856.
  24. Fawzy A, Nitisusanta L, Iqbal K, Daood U, Beng LT, Neo J. Characterization of riboflavin-modified dentin collagen matrix. J Dent Res. 2012;91(11):1049–1054.
  25. Fawzy AS, Nitisusanta LI, Iqbal K, Daood U, Beng LT, Neo J. Chitosan/riboflavin-modified demineralized dentin as a potential substrate for bonding. J Mech Behav Biomed Mater. 2013;17:278–289.
  26. Fawzy AS, Nitisusanta LI, Iqbal K, Daood U, Neo J. Riboflavin as a dentin crosslinking agent: Ultraviolet A versus blue light. Dent Mater. 2012;28(12):1284–1291.
  27. Lehmann N, Debret R, Roméas A, et al. Self-etching increases matrix metalloproteinase expression in the dentin-pulp complex. J Dent Res. 2009;88(1):77–82.
  28. Ishiguro K, Yamashita K, Nakagaki H, Iwata K, Hayakawa T. Identification of tissue inhibitor of metalloproteinases-1 (TIMP-1) in human teeth and its distribution in cementum and dentine. Arch Oral Biol. 1994;39(4):345–349.
  29. Tallant C, Marrero A, Gomis-Rüth FX. Matrix metalloproteinases: Fold and function of their catalytic domains. Biochim Biophys Acta. 2010;1803(1):20–28.
  30. Reis A, Loguercio AD, Manso AP, et al. Microtensile bond strengths for six 2-step and two 1-step self-etch adhesive systems to enamel and dentin. Am J Dent. 2013;26(1):44–50.
  31. Scherrer SS, Cesar PF, Swain MV. Direct comparison of the bond strength results of the different test methods: A critical literature review. Dent Mater. 2010;26(2):e78–e93.
  32. Hass V, Luque-Martinez IV, Gutierrez MF, et al. Collagen cross-linkers on dentin bonding: Stability of the adhesive interfaces, degree of conversion of the adhesive, cytotoxicity and in situ MMP inhibition. Dent Mater. 2016;32(6):732–741.
  33. Hayes S, Boote C, Kamma-Lorger CS, et al. Riboflavin/UVA collagen cross-linking-induced changes in normal and keratoconus corneal stroma. PLoS One. 2011;6(8):e22405.
  34. Liu Y, Tjäderhane L, Breschi L, et al. Limitations in bonding to dentin and experimental strategies to prevent bond degradation. J Dent Res. 2011;90(8):953–968.
  35. Pashley DH, Tay FR, Yiu C, Breschi L, et al. Collagen degradation by host-derived enzymes during aging. J Dent Res. 2004;83(3):216–221.
  36. Zhou J, Tan J, Yang X, Xu X, Li D, Chen L. MMP-inhibitory effect of chlorhexidine applied in a self-etching adhesive. J Adhes Dent. 2011;13(2):111–115.
  37. Cadenaro M, Breschi L, Rueggeberg FA, et al. Effect of adhesive hydrophilicity and curing time on the permeability of resins bonded to water vs ethanol-saturated acid-etched dentin. Dent Mater. 2009;25(1):39–47.