Dental and Medical Problems
2018, vol. 55, nr 2, April-June, p. 125–131
Publication type: original article
The effect of daily intake of green coffee bean extract as compared to Agiolax® on the alveolar bone of albino rats
Wpływ codziennego spożycia wyciągu z ziaren zielonej kawy w odniesieniu do preparatu Agiolax® na kość wyrostka zębodołowego szczurów albinosów
1 Faculty of Oral and Dental Medicine, Cairo University, Egypt
Background. Obesity is a worldwide medical problem in which excess body fat is accumulated in the body. The use of weight loss supplements such as green coffee bean extract and Agiolax has become a common trend among people who want to lose weight in a fast and non-tiring way. As a result of their effect on fluid excretion, both of these products may be expected to have a damaging effect on the alveolar bone.
Objectives. The aim of the present study was to evaluate the histopathalogical effect of green coffee bean extract as compared to Agiolax on the alveolar bone of albino rats.
Material and Methods. Twenty-seven adult male albino rats were randomly assigned to 3 groups. Nine received distilled water daily for 2 months by oral gavage (the control group); the other 2 groups received 1 mg/100 g body weight green coffee bean extract or Agiolax 8 mg/100 g body weight daily for 2 months by oral gavage (the GC and Ag groups, respectively). The alveolar bones were dissected and examined histologically, histomorphometrically and by western blotting.
Results. The bone area percentage and the calcium level in serum were significantly decreased in the GC and Ag groups, while the calcium level in urine was significantly increased in both the experimental groups as compared to the control group. On the other hand, RANKL expression was significantly increased only in the GC group, and the tissue calcium (Ca) level was significantly decreased only in the GC group as compared to the control group.
Conclusion. Long-term oral administration of green coffee bean extract and Agiolax might lead to alveolar bone loss. A greater deleterious effect was caused by green coffee bean extract, as it caused more RANKL expression, significantly reduced Ca level in the tissue and consequently decreased the bone area percentage.
obesity, RANKL, alveolar bone, Agiolax, green coffee bean extract
otyłość, RANKL, kość wyrostka zębodołowego, Agiolax, ekstrakt z ziaren zielonej kawy
- Ellulu M, Abed Y, Rahmat A, Ranneh Y, Ali F. Epidemiology of obesity in developing countries: Challenges and prevention. Glob Epidemiol Obes. 2014;2:2.
- Khan A, Raza S, Khan Y, et al. Current updates in the medical management of obesity. Recent Pat Endocr Metab Immune Drug Discov. 2012;6:117–128.
- Runge CF. Economic consequences of the obese. Diabet. 2007;56:2668–2672.
- Vincent HK, Heywood K, Connelly J, Hurley RW. Weight loss and obesity in the treatment and prevention of osteoarthritis. PMR. 2012;4(Suppl 5):59–67.
- Austin SB, Yu K, Liu SH, Dong F, Tefft N. Household expenditures on dietary supplements sold for weight loss, muscle building, and sexual function: Disproportionate burden by gender and income. Prev Med Rep. 2017;6:236–241.
- Franca AS, Mendonca JCF, Oliveira SD. Composition of green and roasted coffees of different cup qualities. LWT Food Sci Technol. 2005;38:709–715.
- Jeszka-Skowron M, Zgoła-Grześkowiak A, Grześkowiak T. Analytical methods applied for the characterization and the determination of bioactive compounds in coffee. Eur Food Res Technol. 2015;240:19–31.
- Shimoda H, Seki E, Aitani M. Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice. BMC Complement Altern Med. 2006;6:9. doi: 10.1186/1472-6882-6-9
- Vinson JA, Burnham BR, Nagendran MV. Randomized, double-blind, placebo-controlled, linear dose, crossover study to evaluate the efficacy and safety of a green coffee bean extract in overweight subjects. Diabet Metab Syndr Obes. 2012;5:21–27.
- Morales MA, Hernández D, Bustamante S, Bachiller I, Rojas A. Is senna laxative use associated to cathartic colon, genotoxicity, or carcinogenicity? J Toxicol. 2009;2009:287247. doi: 10.1155/2009/287247
- Neims DM, McNeill J, Giles TR, Todd F. Incidence of laxative abuse in community and bulimic populations: A descriptive review. Int J Eat Disord. 1995;17:211–228.
- Zuccaroli J. Treating constipation. South Africa Pharma Assist. 2007;6:6–10.
- Mitchell JM, Mengs U, McPherson S, et al. An oral carcinogenicity and toxicity study of senna (Tinnevelly senna fruits) in the rat. Arch Toxicol. 2006;80:34–44.
- Romero AL, West KL, Zern T, Fernandez ML. The seeds from Plantago ovata lower plasma lipids by altering hepatic and bile acid metabolism in guinea pigs. J Nutr. 2002;132:1194–1198.
- Parfitt AM, Drezner MK, Glorieux FH, et al. Bone histomorphometry: Standardization of nomenclature, symbols, and units. Report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res. 1987;2:595–610.
- Lau DC, Douketis JD, Morrison KM, Hramiak IM, Sharma AM, Ur E. 2006 Canadian clinical practice guidelines on the management and prevention of obesity in adults and children [summary]. CMAJ. 2007;176:1–13.
- Sharpe PA, Granner ML, Conway JM, Ainsworth BE, Dobre M. Availability of weight-loss supplements: Results of an audit of retail outlets in a southeastern city. J Am Diet Assoc. 2006;106:2045–2051.
- Liu SH, Chen C, Yang RS, Yen YP, Yang YT, Tsai C. Caffeine enhances osteoclast differentiation from bone marrow hematopoietic cells and reduces bone mineral density in growing rats. J Orthop Res. 2011;29:954–960.
- Tsuang YH, Sun JS, Chen LT, Sun SC, Chen SC. Direct effects of caffeine on osteoblastic cells metabolism: The possible causal effect of caffeine on the formation of osteoporosis. J Orthop Surg Res. 2006;1:7. doi: 10.1186/1749-799X-1-7
- Wysolmerski JJ. Osteocytes remove and replace perilacunar mineral during reproductive cycles. Bone. 2013;54:230–236.
- da Costa CE, Annels NE, Faaij CM, Forsyth RG, Hogendoorn PC, Egeler RM. Presence of osteoclast-like multinucleated giant cells in the bone and nonostotic lesions of Langerhans cell histiocytosis. J Exp Med. 2005;201:687–693.
- Rapuri PB, Gallagher JC, Kinyamu HK, Ryschon KL. Caffeine intake increases the rate of bone loss in elderly women and interacts with vitamin D receptor genotypes. Am J Clin Nutr. 2001;74:694–700.
- Lacerda SA, Matuoka RI, Macedo RM, Petenusci SO, Campos AA, Brentegani LG. Bone quality associated with daily intake of coffee: A biochemical, radiographic and histometric study. Braz Dent J. 2010;21:199–204.
- Shapses SA, Riedt CS. Bone, body weight, and weight reduction: what are the concerns? J Nutr. 2006;136:1453–1456.
- Bord S, Ireland DC, Beavan SR, Compston JE. The effects of estrogen on osteoprotegerin, RANKL, and estrogen receptor expression in human osteoblasts. Bone. 2003;32:136–141.
- Yenidogan E, Okan I, Kayaoglu HA, et al. Same-day colonoscopy preparation with Senna alkaloids and bisacodyl tablets: A pilot study. World J Gastroenterol. 2014;20:15382–15386.
- Lin XZ, Ma DL, Cui RF. Effects of emodin, sennosides and rheum polysaccharides on free calcium in isolated rat liver cells. Zhongguo Zhong Xi Yi Jie He Za Zhi. 1995;15:419–421.
- He Z, Ma WY, Hashimoto T, Bode AM, Yang CS, Dong Z. Induction of apoptosis by caffeine is mediated by the p53, Bax, and caspase 3 pathways. Cancer Res. 2003;63:4396–4401.
- Bode AM, Dong Z. The enigmatic effects of caffeine in cell cycle and cancer. Cancer Lett. 2007;247:26–39.
- Sohn VR, Giros A, Xicola RM, et al. Stool-fermented Plantago ovata husk induces apoptosis in colorectal cancer cells independently of molecular phenotype. Br J Nutr. 2012;107:1591–602.