Dental and Medical Problems

Dent Med Probl
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Dental and Medical Problems

2017, vol. 54, nr 4, October-December, p. 333–338

doi: 10.17219/dmp/77898

Publication type: original article

Language: English

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Creative Commons BY-NC-ND 3.0 Open Access

Effects of 980 nm diode laser application protocols on the reduction of Enterococcus faecalis intracanal biofilm: An in vitro study

Efekty zastosowania promieniowania laserowego o długości fali 980 nm na redukcję wewnątrzkanałowego biofilmu bakteryjnego Enterococcus faecalis – badania in vitro

Ewa Joanna Prażmo1,A,B,C,D,E,F, Alicja Renata Godlewska2,B,D, Monika Sałkiewicz2,B, Agnieszka Beata Mielczarek1,D,E,F

1 Department of Conservative Dentistry, Medical University of Warsaw, Warsaw, Poland

2 Department of Bacterial Genetics, Faculty of Biology, University of Warsaw, Warsaw, Poland

Abstract

Background. Enterococcus faecalis is associated with a great number of refractory endodontic infections. There is a need to investigate antibacterial properties of laser radiation and create a new alternative technique for root canal disinfection.
Objectives. The purpose of the study was to investigate the effectiveness of a single and repeated highpower diode laser irradiation protocol on the elimination of a 1-week-old Enterococcus faecalis intracanal biofilm.
Material and Methods. A total of 46 single-rooted human teeth were subjected to the in vitro observation. They were chemomechanically prepared, sterilized, infected with a clinically isolated strain of Enterococcus faecalis and subjected to 1-week incubation under microaerobic conditions. The experimental procedures included: a single cycle of 980 nm laser diode irradiation, second application of the same laser protocol, 5.25% NaOCl irrigation, and 2 control groups. Quantitative evaluation of bacterial colonies in the root canals was performed based on the CFU/mL method, after different sterilization methods had been applied.
Results. A statistically significant reduction in the number of intracanal Enterococcus faecalis colonies, after a single and repeated 980 nm diode laser application, was confirmed. The first cycle of laser irradiation eliminated 52.5% of E. faecalis colonies, whereas the second application increased disinfection effectiveness to 87.6%.
Conclusion. The 980 nm diode laser demonstrated statistically significant antibacterial activity. High-power diode laser treatment might be considered as an adjunctive to conventional chemomechanical endodontic treatment.

Key words

Enterococcus faecalis, diode laser, 980 nm, endodontic disinfection

Słowa kluczowe

Enterococcus faecalis, laser diodowy, 980 nm, dezynfekcja endodontyczna

References (31)

  1. Rocas IN, Siqueira JF, Santos KRN. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod. 2004;30:315–320.
  2. Portenier I, Waltimo TMT, Haapasalo M. Enterococcus faecalis – the root canal survivor and star in post treatment disease. Endodont Topics. 2003;6:135–159.
  3. Wang X, Sun Y, Kimura Y, et al. Effect of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation. Photomed Laser Surg. 2005;23:575–581.
  4. Schoop U, Kluger W, Dervisbegovic S, et al. Innovative wavelengths in endodontic treatment. Lasers Surg Med. 2006;38:624–630.
  5. Bergmans L, Moisiadis P, Teughels W, et al. Bactericidal effect of Nd:YAG laser irradiation on some endodontic pathogens ex vivo. Int Endod J. 2006;39:547–557.
  6. Eriksson AR, Albrektsson T. Temperature threshold levels for heat-induced bone tissue injury: A vital-microscopic study in the rabbit. J Prosthet Dent. 1983;50:101–107.
  7. Lane DJ. 16S/ 23S rRNA sequencing. In: Stackebrandt E, Goodfellow M, eds. Nucleic acid techniques in bacterial systematics. New York, NY: John Wiley and Sons; 1991:115–176.
  8. Kishen A, George S, Kumar R. Enterococcus faecalis-mediated biomineralized biofilm formation on root canal dentine in vitro. J Biomed Mater Res. 2006;77A:406–415.
  9. Krause TA, Liewehr FR, Hahn CL. The antimicrobial effect of MTDA, sodium hypochlorite, doxycycline, and citric acid on Enterococcus faecalis. J Endod. 2007;33:28–30.
  10. Zehnder M, Grawehr M, Hasselgren G, et al. Tissue-dissolution capacity and dentine disinfecting potential of calcium hydroxide mixed with irrigating solutions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96:608–613.
  11. Xu Y, Young MJ, Battaglino RA, et al. Endodontic antimicrobial photodynamic therapy: Safety assessment in mammalian cell cultures. J Endod. 2009;35:1567–1572.
  12. Di Renzo M, Ellis TH, Sacher E, et al. A photoacoustic FTIRS study of the chemical modifications of human dentine surfaces: II Deproteination. Biomaterials. 2001;22:793–797.
  13. Farina AP, Cecchin D, Barbizam JV, et al. Influence of endodontic irrigants on bond strength of a self-etching adhesive. Aust Endod J. 2011;37:26–30.
  14. Kaufman AY, Keila S. Hypersensitivity to sodium hypochlorite. J Endod. 1989;15:224–226.
  15. Çaliikan MK, Türkün M, Alper S. Allergy to sodium hypochlorite during root canal therapy: A case report. Int Endod J. 1999;27:163–167.
  16. Romeo U, Palaia G, Nardo A, et al. Effectiveness of KTP laser versus 980 nm diode laser to kill Enterococcus faecalis in biofilms developed in experimentally infected root canals. Aust Endod J. 2015;41:17–23.
  17. Vahdaty A, Ford TRP, Wilson RF. Efficacy of chlorhexidine in disinfecting dentinal tubules in vitro. Endod Dent Traumatol. 1993;9:243–248.
  18. Berutti E, Marini R, Angeretti A. Penetration ability of different irrigants into dentinal tubules. J Endod. 1997;23:725–727.
  19. Marchesan MA, Brugnera-Junior A, Souza-Gabriel AE, et al. Ultrastructural analysis of root canal dentine irradiated with 980 nm diode laser energy at different parameters. Photomed Laser Surg. 2008;26:235–240.
  20. Jhingan P, Sandhu M, Jindal G, et al. An in- vitro evaluation of the 980 nm diode laser irradiation on intra-canal dentine surface and dentinal tubule openings after biomechanical preparation: Scanning electron microscopic study. Indian J Dent. 2006;6:85–90.
  21. Alfredo E, Souza-Gabriel AE, Silva SR, et al. Morphological alterations of radicular dentine pretreated with different irrigations solutions and irradiated with 980 nm diode laser. Microsc Res Tech. 2009;72:22–27.
  22. Coluzzi DJ. An overview of laser wavelengths used in dentistry. Dent Clin North Am. 2000;44:753–761.
  23. Ando Y, Aoki A, Watanabe H, et al. Bactericidal effect of erbum: YAG laser on periodontopatic bacteria. Lasers Surg Med. 1996;19:190–200.
  24. Dworkin M. Endogenous photosensitization in a carotinoidless mutant of Rhodopseudomonas speroides. J Gen Physiol. 1959;41:1099–1112.
  25. Moritz A, Jakolitsch S, Goharkhay K, et al. Morphologic changes correlating to different sensitivities of Escherichia coli and Enterococcus faecalis to Nd:YAG laser irradiation through dentin. Lasers Surg Med. 2000;26:250–261.
  26. Jung H. A Generalized concept for cell killing by heat. Radiat Res. 1986;106:5–72.
  27. Kanumuru NR, Subbaiah R. Bacterial efficacy of Ca(OH)2 against E. faecalis compared with three dental lasers on root canal dentin: An in vitro study. J Clin Diagn Res. 2014;8:ZC135–ZC137.
  28. Mithra NH, Krishna RS, Shishir S, et al. Comparative evaluation of bactericidal effects on Enterococcus faecalis using diode laser irradiation, sodium hypochlorite and chlorhexidine gluconate irrigation: An in vitro study. Oral Health Dent Manag. 2013;12:145–150.
  29. Souza MA, Santos TL, Dall’Magro AK et al. Effectiveness of high-power diode laser associated to chemical auxiliary substance on decontamination of root canal system infected with Enterococcus faecalis: An in vitro study. RFO. 2013;18:288–294.
  30. Grącka-Mańkowska JB, Zarzycka B. Bactericidal efficacy of different diode laser operation modes against Enterococcus faecalis compared with sodium hypochlorite irrigation. Dent. Med. Probl. 2016;53:216–221.
  31. Alfredo E, Marchesan MA, Sousa-Neto MD, et al. Temperature variation at the external root surface during 980 nm diode laser irradiation in the root canal. J Dent. 2008;36:529–534.
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