Dental and Medical Problems

Dent Med Probl
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ISSN 2300-9020 (online)
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Dental and Medical Problems

2017, vol. 54, nr 3, July-September, p. 253–258

doi: 10.17219/dmp/75873

Publication type: original article

Language: English

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Creative Commons BY-NC-ND 3.0 Open Access

Shear bond strength of pit and fissure sealants on permanent teeth after several etching protocols: In vitro study

Wytrzymałość na ścinanie laków szczelinowych w zębach stałych po zastosowaniu kilku protokołów wytrawiania – badania in vitro

Irina Mézquita-Rodrigo1,B,C,D,F, Rosalía Contreras-Bulnes1,A,B,C,D,E,F, Laura Emma Rodríguez-Vilchis1,A,B,C,D,E,F, Rogelio José Scougall-Vilchis1,A,C,E,F, Ulises Velazquez-Enriquez1,C,E,F, Claudia Centeno-Pedraza1,B,F

1 Dental and Advanced Studies Research Center, Faculty of Dentistry Autonomous Unversity State of Mexico, Toluca, Mexico


Background. The pit and fissure areas are 8 times more vulnerable to caries; consequently, sealants have been proven to be efficient. Acid etching is the standard procedure for enamel etching; however, it leaves a demineralized surface. Laser Er:YAG is a good etching alternative due to its properties.
Objectives. The aim of this study was to evaluate the effect of different conditioning agents on shear bond strength (SBS) of sealants in permanent teeth.
Material and Methods. A total of 156 third molar samples were divided into 6 groups (n = 25 each). Treated as follows: GI acid etching (35% phosphoric acid), GII Er:YAG laser, GIII self-etching adhesive, GIV acid + Er:YAG laser, GV Er:YAG laser + acid and GVI Er:YAG laser + self-etching adhesive. Er:YAG laser irradiation was at 19.1 J/cm2, water spraying at 5.0 mL/min. Sealant blocks were made and bonded on the enamel with a sealant in order to perform SBS test and adhesive remnant index (ARI). Samples were stored (37°C, 24 h), tested, and statistically analyzed with a one way ANOVA, Kruskall-Wallis and Mann-Whitney U. SEM images were taken to assess the conditioning methods (n = 6).
Results. Er:YAG + self-etching group showed the lowest range. The highest values were observed in GI, GII and GIII, no significant differences were found among them. The largest amount of adhesive remnant was found in group V.
Conclusion. Phosphoric acid (35%), self-etching as well as 35% phosphoric acid + Er:YAG laser protocols showed a better shear bond strength for pit and fissure sealant adhesion. Er:YAG laser + self-etching protocol is not recommended for this procedure, due to the lack of adequate adhesive strength.

Key words

shear bond strength, pit and fissure sealants, several etching protocols DOI

Słowa kluczowe

wytrzymałość na ścinanie, laki szczelinowe, techniki wytrawiania szkliwa

References (30)

  1. Ahovuo-Saloranta A, Forss H, Walsh T, et al. Sealants for preventing dental decay in the permanent teeth. Cochrane Database Syst Rev. 2013;28:1–32.
  2. Perhavec T, Gorkič A, Bračun D, Diaci J. A method for rapid measurement of laser ablation rate of hard dental tissue. Opt Laser Technol. 2009;41:397–402.
  3. Cartensen W. The effects of different phosphoric acid concentrations on surface enamel. Angle Orthod. 1992;62:51–58.
  4. Oho T, Morioka T. A possible mechanism of acquired acid resistance of human dental enamel by laser irradiation. Caries Res. 1990;24:86–92.
  5. Fried D, Featherstone JDB, Visuri SR, Seka W, Walsh JT. The caries inhibition potential of Er:YAG and Er:YSGG laser radiation. Lasers in Dentistry II. Proceedings of SPIE, 1996; 2672:73–78.
  6. Liu YY, Hsu CYS. Laser-induced compositional changes on enamel: A FT-Raman study. J Dent. 2007;35:226–230.
  7. Borsatto MC, Corona SA, Dibb RG, Ramos RP, Pécora JD. Microleakage of a resin sealant after acid-etching, Er:YAG laser irradiation and air-abrasion of pits and fissures. J Clin Laser Med Surg. 2001;19:83–87.
  8. Ciucchi, P, Neuhaus KW, Emerich M, Peutzfeldt A, Lussi A. Evaluation of different types of enamel conditioning before application of a fissure sealant. Lasers Med Sci. 2015;30:1–9.
  9. Yung F, Gutknecht N, Franzen R, Fischer H. Shear strength of composite bonded to Er:YAG laser-prepared enamel: An in vitro comparative study. Lasers Med Sci. 2013;28:879–889.
  10. Lupi-Pégurier L, Bertrand MF, Genovese O, Rocca JP, Muller-Bolla M. Microleakage of resin-based sealants after Er:YAG laser conditioning. Lasers Med Sci. 2007;22:183–188.
  11. Lopes RM, Trevelin LT, da Cunha SR, et al. Dental adhesion to erbium-lased tooth structure: A review of the literature. Photomed Laser Surg. 2015;33:393–403.
  12. Shahabi S, Bagheri HG, Ramazini K. Tensile bond strength of sealants following Er:YAG laser etching compared to acid etching in permanent teeth. Lasers Med Sci. 2012;27:371–375.
  13. Bader C, Krejci I. Indications and limitations of Er:YAG laser applications in dentistry. Am J Dent. 2006;19:178–186.
  14. Esteves-Oliveira M, Zezell DM, Apel C, et al. Bond strength of self-etching primer to bur cut, Er, Cr: YSGG, and Er:YAG lased dental surfaces. Photomed Laser Surg. 2007;25:373–380.
  15. Chimello-Sousa DT, de Souza AE, Chinelatti MA, Pécora JD, Palma-Dibb RG, Milori Corona SA. Influence of Er:YAG laser irradiation distance on the bond strength of a restorative system to enamel. J Dent. 2006;34:245–251.
  16. Colucci V, de Souza Gabriel AE, Scatolin RS, Serra MC, Corona SA. Effect of Er:YAG laser on enamel demineralization around restorations. Lasers Med Sci. 2015;30:1175–1181.
  17. Díaz-Monroy JM, Contreras-Bulnes R, Olea-Mejía OF, Rodríguez-Vilchis LE, Sánchez-Flores I. Morphological changes produced by acid dissolution in Er: YAG laser irradiated dental enamel. Microsc Res Tech. 2014;77:410–414.
  18. Kim JH, Kwon OW, Kim HI, Kwon YH. Acid resistance of erbium-doped yttrium aluminum garnet laser-treated and phosphoric acid-etched enamels. Angle Orthod. 2006;76:1052–1056.
  19. Mathew A, Reddy NV, Sugumaran DK, Peter J, Shameer M, Dauravu LM. Acquired acid resistance of human enamel treated with laser (Er:YAG laser and CO2 laser) and acidulated phosphate fluoride treatment: An in vitro atomic emission spectrometry analysis. Contemp Clin Dent. 2013;4:170–175.
  20. Güçlü ZA, Dönmez N, Tüzüner T, Odabaş ME, Hurt AP, Coleman NJ. The impact of Er:YAG laser enamel conditioning on the microleakage of a new hydrophilic sealant-UltraSeal XT® hydro™. Lasers Med Sci. 2016;31:705–711.
  21. Alcántara-Galeana M, Contreras-Bulnes R, Rodríguez-Vilchis L, et al. Microhardness, structure, and morphology of primary enamel after phosphoric acid, self-etching adhesive, and Er:YAG laser etching. Int J Optics, 2017;ID7634739.
  22. Wanderley RL, Monghini EM, Pecora JD, Palma-Dibb RG, Borsatto MC. Shear bond strength to enamel of primary teeth irradiated with varying Er:YAG laser energies and SEM examination of the surface morphology: An in vitro study. Photomed Laser Surg. 2005;23:260–267.
  23. Correa-Alfonso AM, Ciconne-Nogueira JC, Pécora JD, Palma-Dibb RG. In vitro assessment of laser efficiency for caries prevention in pits and fissures. Microsc Res Tech. 2012;75: 245–252.
  24. Contreras-Bulnes R, Scougall-Vilchis RJ, Rodríguez-Vilchis LE, Centeno-Pedraza C, Olea-Mejía OF, Alcántara-Galeana Mdel C Z. Evaluation of self-etching adhesive and Er:YAG laser conditioning on the shear bond strength of orthodontic brackets. SciWorldJ. 2013;ID719182.
  25. Vicente A, Bravo LA, Romero M. Influence of a nonrinse conditioner on the bond strength of brackets bonded with a resin adhesive system. Angle Orthod. 2005;75:400–405.
  26. Tecco S, Traini T, Caputi S, Festa F, de Luca V, D’Attilio M. A new one-step dental flowable composite for orthodontic use: An in vitro bond strength study. Angle Orthod. 2005;75:672–677.
  27. Penmetsa RKR, Sri Rekha A, Poppuri KC, Sai Prashanth P, Garapati S. An in vitro evaluation of antibacterial properties of self etching dental adhesive systems. J Clin Diagn Res. 2014;8:1–5.
  28. Łukomska-Szymanska M, Konieczka M, Zarzycka B, Lapinska B, Grzegorczyk J, Sokolowski J. Antibacterial activity of commercial dentine bonding systems against E. faecalis – flow cytometry study. Materials, 2017;10:481.
  29. Łukomska-Szymanska M, Olbert-Sieroszewska V, Zurawska‑Olszewska J, Szczerba I, Krzeminski Z, Sokołowski J. Antibacterial properties of 6th generation bonding systems. Dent Med Probl. 2010;47:304–308 [in Polish].
  30. Chen ML, Ding JF, He YJ, Chen Y, Jiang QZ. Effect of pretreatment on Er:YAG laser-irradiated dentin. Lasers Med Sci. 2015;30:753–759.