Dental and Medical Problems

Dent Med Probl
Index Copernicus (ICV 2020) – 128.41
MEiN – 70 pts
CiteScore (2021) – 2.0
JCI – 0.22
Average rejection rate (2021) – 81.35%
ISSN 1644-387X (print)
ISSN 2300-9020 (online)
Periodicity – quarterly

Download PDF

Dental and Medical Problems

2016, vol. 53, nr 3, July-September, p. 338–344

doi: 10.17219/dmp/62973

Publication type: original article

Language: English

Download citation:

  • BIBTEX (JabRef, Mendeley)
  • RIS (Papers, Reference Manager, RefWorks, Zotero)

Creative Commons BY-NC-ND 3.0 Open Access

Usage of Low Level Laser Biostimulation and Platelet Rich Fibrin in Bone Healing: Experimental Study

Zastosowanie lasera biostymulacyjnego z osoczem bogatopłykowym w gojeniu się kości

Khaled Atef El-Hayes1,A,B,D,E,F, Ahmed Abbas Zaky2,A,B,D,E,F, Zeinab Amin Ibrahim3,B,C,D,E,F, Ghada Farouk Ahmed Allam4,A,B,C,D,E,F, Mohamed Farouk Allam5,A,B,C,D,E,F

1 Oral and Maxillofacial Surgery, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt

2 Medical Laser Applications, National Institute of Laser Enhanced Sciences, Cairo University, Cairo, Egypt

3 Oral Biology, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt

4 Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt

5 Department of Preventive Medicine and Public Health, Faculty of Medicine, University of Cordoba, Cordoba, Spain


Background. Experimental studies have shown that low level laser therapy (LLLT) has a positive local biostimulative effect in the early stage of bone healing. Platelet rich fibrin (PRF) also has been shown to be effective in the treatment of intrabony periodontal defects.
Objectives. The objective of our experimental study was to demonstrate the combined effects of LLLT and PRF on bone healing.
Material and Methods. Our experimental study was done over 80 bony cavities in 20 adult male rabbits, aged 12 months. An incision was made for exposure of the femur bone of all rabbits. Then, by using a large, round surgical bur, a perforated hole was made in the femur. The cavities induced in these rabbits were divided into 4 groups: The control group which was neither subjected to any laser irradiation nor filled with any bone substitute (group I); The bony defects were filled with PRF (group II); The cavities were subjected to low level laser (LLL) for biostimulation (group III); The cavities were subjected to LLL for biostimulation then were filled with PRF (group IV). Histological assessments of the four groups were done using a hematoxylin and eosin stain. Statistical analysis was done using ANOVA and Bonferroni tests for comparisons between the four groups.
Results. The area percentage of the newly formed bone in group IV was significantly higher than the other three groups. The area percentage of the newly formed bone in group III is significantly higher than group II.
Conclusion. LLLT could induce bone formation in the bone defect at a faster rate than PRF. However, a combination of both LLLT and PRF as treatment modalities could induce bone formation in the bone defect more than that of LLLT or PRF alone.

Key words

low level laser, biostimulation, experimental study, bone healing, platelet rich fibrin

Słowa kluczowe

laser niskoenergetyczny, biostymulacja, badanie eksperymentalne, gojenie się kości, osocze bogatopłytkowe

References (28)

  1. Surendranath P., Arjunkumar R.: Low level laser therapy – a review. J. Dent. Med. Sci. 2013, 12, 56–59.
  2. Mester E., Mester AF., Mester A.: The biomedical effects of laser application. Lasers Surg. Med. 1985, 5, 31–39.
  3. Garavello-Freitas I., Baranauskas V., Joazeiro P.P., Padovani C.R., DalPai-Silva M., da Cruz-Hofling M.A.: Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J. Photochem. Photobiol. B. 2003, 70, 81–89.
  4. Pretel H., Lizarelli R.F., Ramalho L.T.: Effect of low-level laser therapy on bone repair: Histological study in rats. Lasers Surg. Med. 2007, 39, 788–796.
  5. Ribeiro D.A., Matsumoto M.A.: Low-level laser therapy improves bone repair in rats treated with anti-inflammatory drugs. J. Oral Rehabil. 2008, 35, 925–933.
  6. Kreisner P.E., Blaya D.S., Gaiao L., Maciel-Santos M.E., Etges A., Santana-Filho M.: Histological evaluation of the effect of low-level laser on distraction osteogenesis in rabbit mandibles. Med. Oral Patol. Oral Cir. Bucal. 2010, 15, e616–618.
  7. Gordjestani M., Dermaut L., Thierens H.: Infrared laser and bone metabolism: A pilot study. Int. J. Oral Maxillofac. Surg. 1994, 23, 54–56.
  8. David R., Nissan M., Cohen I., Soudry M.: Effect of low-power He-Ne laser on fracture healing in rats. Lasers Surg. Med. 1996, 19, 458–464.
  9. Ebrahimi T., Moslemi N., Rokn A., Heidari M., Nokhbatolfoghahaie H., Fekrazad R.: The influence of low intensity laser therapy on bone healing. J. Dent. (Tehran) 2012, 9, 238–248.
  10. Naik B., Karunakar P., Jayadev M., Marshal V.R.: Role of platelet rich fibrin in wound healing: A critical review. J. Conserv. Dent. 2013, 16, 284–293.
  11. Choukroun J., Diss A., Simonpieri A., Girard M.O., Schoeffler C., Dohan S.L.: Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 2006, 101, 299–303.
  12. Del Fabbro M., Gallesio G., Mozzati M.: Autologous platelet concentrates for bisphosphonate-related osteonecrosis of the jaw treatment and prevention. A systematic review of the literature. Eur. J. Cancer. 2015, 51, 62–74.
  13. Davis V.L., Abukabda A.B., Radio N.M., Witt-Enderby P.A., Clafshenkel W.P., Cairone J.V., Rutkowski J.L.: Platelet-rich preparations to improve healing. Part II: Platelet activation and enrichment, leukocyte inclusion, and other selection criteria. J. Oral Implantol. 2014, 40, 511–521.
  14. Kumar R.V., Shubhashini N.: Platelet rich fibrin: A new paradigm in periodontal regeneration. Cell Tissue Bank, 2013, 14, 453–463.
  15. Borie E., Oliví D.G., Orsi I.A., Garlet K., Weber B., Beltrán V., Fuentes R.: Platelet-rich fibrin application in dentistry: A literature review. Int. J. Clin. Exp. Med. 2015, 8, 7922–7929.
  16. Acar A.H., Yolcu Ü., Altındiş S., Gül M., Alan H., Malkoç S.: Bone regeneration by low-level laser therapy and low-intensity pulsed ultrasound therapy in the rabbit calvarium. Arch. Oral Biol. 2016, 61, 60–65.
  17. Richardson V.C.G.: The musculoskeletal system. [In:] Rabbits: Health, Husbandry and Diseases. Ed.: Richardson V.C.G. Wiley-Blackwell 2000, 108–114.
  18. Elgendy E.A., Abo Shady T.E.: Clinical and radiographic evaluation of nanocrystalline hydroxyapatite with or without platelet-rich fibrin membrane in the treatment of periodontal intrabony defects. J. Indian Soc. Periodontol. 2015, 19, 61–65.
  19. Hawkins D.H., Abrahamse H.: The role of laser influence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium-neon laser irradiation. Lasers Surg. Med. 2006, 38, 74–83.
  20. Batista J.D., Sargenti-Neto S., Dechichi P., Rocha F.S., Pagnoncelli R.M.: Low-level laser therapy on bone repair: Is there any effect outside the irradiated field? Lasers Med. Sci. 2015, 30, 245–249.
  21. Fávaro-Pípi E., Ribeiro D.A., Ribeiro J.U., Bossini P., Oliveira P., Parizotto N.A., Tim C., de Araújo H.S., Renno A.C.: Low-level laser therapy induces differential expression of osteogenic genes during bone repair in rats. Photomed. Laser Surg. 2011, 29, 311–317.
  22. Silva Júnior A.N., Pinheiro A.L., Oliveira M.G., Weismann R., Ramalho L.M., Nicolau R.A.: Computerized morphometric assessment of the effect of low-level laser therapy on bone repair: An experimental animal study. J. Clin. Laser Med. Surg. 2002, 20, 83–87.
  23. De Almeida A.L., Medeiros I.L., Cunha M.J., Sbrana M.C., de Oliveira P.G., Esper L.A.: The effect of lowlevel laser on bone healing in critical size defects treated with or without autogenous bone graft: An experimental study in rat calvaria. Clin. Oral Implants Res. 2014, 25, 1131–1136.
  24. Da Silva R.V., Camilli J.A.: Repair of bone defects treated with autogenous bone graft and low-power laser. J. Craniofac. Surg. 2006, 17, 297–301.
  25. Nicola R.A., Jorgetti V., Rigau J., Pacheco M.T., dos Reis L.M., Zângaro R.A.: Effect of low-power GaAlAs laser (660 nm) on bone structure and cell activity: An experimental animal study. Lasers Med. Sci. 2003, 18, 89–94.
  26. Ozawa Y., Shimizu N., Kariya G., Abiko Y.: Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone, 1998, 22, 347–354.
  27. Kazem Shakouri S., Soleimanpour J., Salekzamani Y., Oskuie M.R.: Effect of low-level laser therapy on the fracture healing process. Lasers Med. Sci. 2010, 25, 73–77.
  28. Kucharska E., Batko B.: Biostimulating laser therapy as one method of treating bone and joint diseases. Przegl. Lek. 1997, 54, 112–124 [in Polish].