Dental and Medical Problems

Dent Med Probl
Impact Factor (IF 2023) – 2.7
Journal Citation Indicator (JCI 2023) - 1.06
Scopus CiteScore (2023) – 4.0 (CiteScore Tracker – 4.9)
Index Copernicus (ICV 2023) – 181.00
MNiSW – 70 pts
ISSN 1644-387X (print)
ISSN 2300-9020 (online)
Periodicity – bimonthly


 

Download original text (PL)

Dental and Medical Problems

2012, vol. 49, nr 2, April-June, p. 299–304

Publication type: review

Language: Polish

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

Cytotoksyczność współcześnie stosowanych systemów wiążących – na podstawie piśmiennictwa

Cytotoxicity of Current Adhesive Systems – Based on Literature Review

Elżbieta Jodkowska1,A-F

1 Zakład Stomatologii Zachowawczej Instytutu Stomatologii Warszawskiego Uniwersytetu Medycznego

Streszczenie

Wszystkie współcześnie stosowane materiały dentystyczne w różnym stopniu uwalniają substancje do środowiska jamy ustnej. Znajomość podstawowych reguł ich zastosowania pozwoli zapobiec temu niepożądanemu procesowi lub przynajmniej go ograniczyć. Na podstawie piśmiennictwa zwrócono uwagę na możliwość wystąpienia reakcji ubocznych środowiska jamy ustnej na systemy wiążące. Badania in vitro i in vivo potwierdziły, że uwalniające się monomery powodują uszkodzenia chemiczne w hodowlach komórkowych i komórkach miazgi, penetrując przez tkankę zębinową, zakłócają metabolizm komórek. Oprócz molekularnej cytotoksyczności dużych stężeń monomerów, małe stężenia mogą zaburzyć metabolizm, homeostazę komórkową, indukować apoptozę lub zatrzymać cykl komórkowy. Żywice Bis-GMA, HEMA, TEGDMA zmieniają ekspresję genów, których produkty biorą udział w regulacji wzrostu komórki, proliferacji i zróżnicowaniu, reaplikacji i naprawie DNA.

Abstract

All dental biomaterials release substances to the oral cavity with varying degree. Knowing the basic rules of internal use enables to prevent such adverse reactions, or at least minimize them. Based on literature review, a potential occurrence of side effects of the adhesive systems on the oral cavity, is described. In vitro and in vivo studies have confirmed that released monomers cause chemical damage in cells cultures and in pulp cells. Penetrating through tooth tissue monomers disrupt meous monomers at high concentration, low concentration can have deleterious effect on the metabolism, cellular homeostasis, can also trigger apoptosis or cell cycle delay. Bis-GMA, HEMA, TEGDMA can induce the expression of the genes whose products are involved in regulation of cell growth, gene expression, proliferation and differentiation, reapplication and DNA repair.

Słowa kluczowe

zębinowe systemy wiążące, cytotoksyczność, zębina

Key words

dentinal adhesive systems, cytotoxicity, dentin

References (35)

  1. Huang F.M., Chang Y.: Cytotoxicity of dentin-bonding agents on human pulp cells in vitro. Int. Endod. J. 2002, 35, 905–909.
  2. Carvalho R.M., Mendoca J.S., Santiago S.L., Silveira R.R., Garcia F.C.P., Tay F.R.: Effects of HEMA/solvent combinations on bond strength to dentin. J. Dent. Res. 2003, 82, 597–601.
  3. Van Meerbeck B., Vargas S., Inoue S., Yoshida Y., Peumans M., Lambrechts P.: Adhesives and cements to promote preservation dentistry. Oper. Dent. 2001, 26, S119–S144.
  4. Van Meerbeck B., De Munck J., Yoshida Y., Inoue S., Vargas M., Vijay P.: Buonocore memorial lecture: adhesion to enamel and dentin:current status and future challenges. Oper. Dent. 2003, 28, 215–235.
  5. Nakabayashi N., Kojima K., Masuhara E.: The promotion of adhesion by the infiltration of monomer into tooth substrates. J. Biomed. Mater. Res. 1982, 16, 1240–1243.
  6. Sauro S., Pashley D.H., Montanari M., Chersoni S., Carvalho R.M., Toledano M.: Effect of simulated pulpal pressure on dentin permeability and adhesion of self-etch adhesives. Dent. Mater. 2007, 23, 705–713.
  7. Tay F.R., Frankenberg R., Krejci I., Bouillaguet S., Pashley D.H., Carvalho R.M.: Single-bottle adhesives behave as permeable membranes after polymerization. In vivo evidence. J. Dent. 2004, 32, 611–621.
  8. Dickens S.H., Stansbury J.W., Choć K.M., Floyd C.J.E.: Photopolymerization kinetics of methacrylate dental resins. Macromolec. 2003, 36, 6043–6053.
  9. Michelsen V.B, Lygre H., Shalevik R., Tveit A.B., Solheim E.: Identification of organic eluates from four polymerbased dental filling materials. Eur. J. Oral Sci. 2003, 111, 263–271.
  10. Moszner N., Salz V.: New developments of polymeric dental composites. Prog. Polym. Sci. 2001, 26, 535–576.
  11. Floyd C.J.E., Dickens S.H.: Network structure of bis-GMA and UDMA-based resin systems. Dent. Mater. 2006, 22, 1143–1149.
  12. Nocca G., De Palma F., Minucci A., De Sole P., Martorana G.E., Calla C.: Alternations of energy metabolism and glutathisone levels of HL-60 cells inducted by methacrylates present in composite resins. J. Dent. 2007, 35, 187–194.
  13. Costa C.A.S., Nascimento A.B.I., Teixeira H.M.: Response of human pulps following acid conditioning and application of a bonding agent in deep cavities. Dent. Mater. 2002, 18, 543–551.
  14. Postek-Stefańska L.: Zgodność biologiczna materiałów kompozytowych i pośrednich systemów łączących w badaniach in vitro i in vivo. Rozprawa habilitacyjna. Śląska Akademia Medyczna, Katowice 2001.
  15. Hanks C.T., Craig R.G., Diehl M.L., Pashley D.H.: Cytotoxicity of dental composite and other materials in a new in vitro device. J. Oral Pathol. Med. 1988, 17, 396–403.
  16. Bouillaquet S., Virgillito M., Wataha J., Ciucchi B., Holz J.: The influence of dentine permeability on cytotixicity of four dentine bonding systems, in vitro. J. Oral Rehabil. 1998, 25, 45–51.
  17. Ratanasathien W., Wataha J.C., Hanks C.T., Dennison J.B.: Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J. Dent. Res. 1995, 74, 1602–1606.
  18. Hanks C.T., Strawn S.E., Wataha J.C., Craig R.G.: Cytotoxic effects of resin components on cultured mammalian fibroblasts. J. Dent. Res. 1991, 70, 1450–1455.
  19. Wataha J.C., Hanks C.T., Strawn S.E., Fat J.C.: Cytotoxicity of components of resin and other dental restorative materials. J. Oral Rehabil. 1994, 21, 453–462.
  20. Yoshii E.: Cytotoxic effects of acrylates and methacrylates relationship of monomer structures and cytotoxicity. J. Biomed. Mater. Res. 1997, 37, 4, 517–524.
  21. Porto J.C.C.M., Oliveira D.C., Raele R.A., Ribas K.H.S., Montes M.A.J.R., De Castro C.M.M.B.: Cytotoxicity of current adhesive systems: In vitro testing on cell cultures of primary murine macrophages. Dent. Mater. 2011, 27, 221–228.
  22. Huang F.M., Chang Y.: Cytotoxicity of dentin-bonding agents on human pulp cells in vitro. Int. Endod. J. 2002, 35, 905–909.
  23. Pashley D.H.: Consideration of dentin permeability in cytotoxicity testing. Int. Endod. J. 1988, 21, 143–154.
  24. Chen R.S., Lin C.C., Tseng W.Y., Jeng J.H., Lin C.P.: Cytotoxicity of three dentin bonding agents of human dental pulp cells. J. Dent. 2003, 31, 223–229.
  25. Lin N.J., Bailey L.O., Becker M.L., Washburn N.R., Heneterson L.A.: Macrophage response to methacrylate conversion using a gradient approach. Acta Biomater. 2007, 3, 163–173.
  26. Miletic V., Santini A., Trkulja J.: Quantification of monomer elation and carbon-carbon double bonds in dental adhesive systems using HPLC and micro-Raman spectroscopy. J. Dent. 2009, 37, 177–184.
  27. Tay F.R., Pashley D.H.: Aggressiveness of contemporary self-etching systems I. Depth of penetration beyond smear layers. Dent. Mater. 2001, 17, 296–308.
  28. Cadenaro M., Antoniolli F., Sauro S., Tay F.R., Di Leonarda R., Prati C.: Degree of conversion and permeability of dental adhesives. Eur. J. Oral Sci. 2005, 113, 525–530.
  29. Kostoryz E.L., Eick J.D., Chappelow C.C., Glaros A.G., Wetmore L., Yourtee D.M.: In vitro effect of lightcure dental adhesive on IL-6 release from LPS-stimulated and unstimulated macrophages. J. Biomed. Mater. Res. A. 2003, 65, 89–94.
  30. Lefebvre C.A., Schuster G., Rueggeberg F.A., Tamareselvy K., Kneorschild K.L: Responses of oral epithelial cells to dental resin components. J. Biomater. Sci. Polym. Ed. 1996, 7, 965–976.
  31. Kosforyz E.L., Samuelsen J.T., Holme J.A., Becher R., Karlsson S., Morisbak E., Dahl J.E.: HEMA reduces cell proliferation and induces apoptosis in vitro. Dent. Mater. 2008, 24, 134–140.
  32. Hamid A., Hume W.R.: A study of component release from resin pit and fissure sealant in vitro. Dent. Mater. 1997, 13, 98–102.
  33. Hamid A., Okomoto A., Iwaka M., Hume W.R.: Component realease from light-activated glass-ionomer and compomer cements. J. Oral Rehabil. 1998, 25, 94–99.
  34. Volk J., Zieman C., Leyhausen G., Geursten W.: Non-irradiated camphorquinone induces DNA damage in human gingival fibroblasts. Dent. Mater. 2009, 22: doi 10.1016/jdental 2009.07.009
  35. Rueggeberg F.A., Margeson D.H.: The effects of oxygen inhibition on an unfield /filled composite system. J. D ent. Res. 1990, 69, 1652–1698.
© Wroclaw Medical University