Dental and Medical Problems

Dent Med Probl
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Dental and Medical Problems

2011, vol. 48, nr 4, October-December, p. 474–480

Publication type: original article

Language: English

License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

Elastase Concentration in Saliva in Patients with Chronic Periodontitis

Stężenie elastazy w ślinie pacjentów z przewlekłym zapaleniem przyzębia

Małgorzata Nędzi-Góra1,, Renata Górska1,

1 Department of Periodontology and Oral Diseases Warsaw Medical University, Poland

Abstract

Background. The traditional diagnosis of periodontal disease is based on clinical and radiological examination. Ideal diagnostics should be based not only on clinical parameters of periodontal tissues, but also on biochemical indices which could indicate a potential progression of the disease. Elastase is one of numerous proteolytic enzymes released by neutrophilic granulocytes in the course of periodontitis.
Objectives. The aim of the study was to determine the concentration of elastase in saliva in patients with chronic periodontitis compared to healthy individuals.
Material and Methods. The enzyme-linked immunosorbent assay method was employed to determine the concentration of elastase in saliva in patients with chronic periodontitis and with pocket depth (PD) ≥ 4 mm and PD < 4 mm, as well as in saliva of healthy individuals.
Results. A significantly higher concentration of elastase was observed in patients with periodontitis compared to healthy individuals. Also a significant difference in elastase concentration in saliva was observed between the PD ≥ 4 mm and PD < 4 mm groups and between the PD ≥ 4 mm and control groups as well as no statistically significant differences were observed between the PD < 4 mm and control groups.
Conclusion. The elastase concentration in saliva can be considered as one of biochemical indicators of severity of periodontitis.

Streszczenie

Wprowadzenie. Tradycyjne rozpoznanie choroby przyzębia wykorzystuje badanie kliniczne i radiologiczne. Idealna diagnostyka powinna uwzględniać nie tylko kliniczne wskaźniki stanu tkanek przyzębia, ale również wskaźniki biochemiczne, które mogłyby wskazywać na potencjalną progresję choroby. Elastaza jest jednym z wielu enzymów proteolitycznych uwalnianych przez granulocyty obojętnochłonne w przebiegu zapalenia przyzębia.
Cel pracy. Określenie stężenia elastazy w ślinie pacjentów z przewlekłym zapaleniem przyzębia w porównaniu do osób ze zdrowym przyzębiem.
Materiał i metody. Metodą immunoenzymatyczną oznaczono stężenie elastazy w ślinie pacjentów z przewlekłym zapaleniem przyzębia i kieszonkami przyzębnymi o głębokości (PD) ≥ 4 mm oraz PD < 4 mm oraz w ślinie osób zdrowych.
Wyniki. Stwierdzono znamiennie wyższe stężenie elastazy w ślinie pacjentów z zapaleniem przyzębia w porównaniu do osób zdrowych. Wykazano również istotność różnic w stężeniu elastazy w ślinie między grupami PD ≥ 4 mm i P D < 4 mm oraz PD ≥ 4 mm i kontrolną oraz brak różnic statystycznie istotnych między grupami PD < 4 mm i kontrolną.
Wnioski. Stężenie elastazy w ślinie można brać pod uwagę jako jeden ze wskaźników biochemicznych stopnia nasilenia zapalenia przyzębia.

Key words

chronic periodontitis, elastase, saliva

Słowa kluczowe

przewlekłe zapalenie przyzębia, elastaza, ślina

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