Dental and Medical Problems

Dent Med Probl
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Dental and Medical Problems

2011, vol. 48, nr 3, July-September, p. 319–324

Publication type: original article

Language: English

The Molecular Prognosis on Bone Osteolysis in Epulides

Molekularne prognozowanie osteolizy kości w nadziąślakach

Iwona Niedzielska1,, Bartłomiej Mrowiec2,

1 Department of Maxillofacial Surgery, Medical University of Silesia, Poland

2 Dental Practice “Polmedico”

Abstract

Background. Epuli are the most frequent gingival tumours. The lesions are often associated with bone osteolysis which necessitates more extensive surgery or removal of tumour-adjacent teeth. Recurrence, related to non-radical tumour excision, is also common. No literature data have been found concerning molecular markers responsible for the processes of osteolysis and recurrence.
Objectives. The aim of the study is molecular prognosis of bone osteolysis in epuli patients.
Material and Methods. Molecular investigations by RT-PCR were carried out on 210 biopsies collected from 70 epulus lesions. Depending on the diagnosis of bone osteolysis/lesion recurrence, expression of genes, i.e., BCL-2, BAX, H3 histone, IFNγ and its IFNγR1, IFNγR2 receptors was investigated.
Results. Recurrence and osteolysis were observed when IFNγR1/IFNγR2 quotient < 0.5. Apoptosis and proliferation genes expression did not show any significant changes.
Conclusion. When IFNγR1/IFNγR2 < 0.5, recurrence and bone osteolysis can be predicted in epuli patients.

Streszczenie

Wprowadzenie. Nadziąślaki należą do grupy najczęściej spotykanych guzów dziąsłowych jamy ustnej. Zmianie często towarzyszy osteoliza kości, która wymaga rozległego zabiegu chirurgicznego lub usunięcia guza razem z sąsiadującym zębem. Często dochodzi do nawrotu związanego z nieradykalnym wycięciem guza. W piśmiennictwie brakuje doniesień na temat molekularnych markerów odpowiedzialnych za osteolizę i nawrót w przebiegu nadziąślaków.
Cel pracy. Propozycja prognozowania osteolizy kości w przebiegu nadziąślaków.
Materiał i metody. Badania molekularne z użyciem RT-PCR były prowadzone na podstawie pobranych 210 badań histopatologicznych z 70 nadziąślaków. W zależności od stwierdzanej osteolizy kości/zmian nawrotowych oceniano ekspresję genów, np. BCL-2 BAX, histon H3, IFNγ i jego receptorów IFNγR1, IFNγR2.
Wyniki. Nawrót i osteolizę obserwowano, gdy iloraz IFNγR1/IFNγR2 był mniejszy od 0,5. Nie stwierdzono istotnych zmian w ekspresji genów odpowiedzialnych za apoptozę i proliferację.
Wnioski. Gdy IFNγR1/IFNγR2 < 0,5, można przewidywać nawrót i osteolizę kości u osób z nadziąślakami.

Key words

epulus, RT-PCR, IFNγR1, IFNγR2, IFNγ

Słowa kluczowe

nadziąślak, RT-PCR, IFNγR1, IFNγR2, IFNγ

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